Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition.
In: Journal of cell science, Jg. 118 (2005-03-01), Heft Pt 5, S. 873-87
academicJournal
Zugriff:
Epithelium-to-mesenchyme transitions (EMTs) are characterized by morphological and behavioral changes in cells. During an EMT, E-cadherin is downregulated while N-cadherin is upregulated. The goal of this study was to understand the role cadherin switching plays in EMT using a classical model system: transforming growth factor beta1 (TGF-beta1)-mediated EMT in mammary epithelial cells. We showed that stress fibers and focal adhesions are increased, and cell-cell junctions are decreased in response to TGF-beta1. Moreover, these changes were reversible upon removal of TGF-beta1. Downregulation of E-cadherin and upregulation of N-cadherin were both transcriptional. Neither experimental knockdown nor experimental overexpression of N-cadherin interfered with the morphological changes. In addition, the morphological changes associated with EMT preceded the downregulation of E-cadherin. Interestingly, TGF-beta1-induced motility in N-cadherin-knockdown cells was significantly reduced. Together, these data suggest that cadherin switching is necessary for increased motility but is not required for the morphological changes that accompany EMT.
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Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition.
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Autor/in / Beteiligte Person: | Maeda, M ; Johnson, KR ; Wheelock, MJ |
Zeitschrift: | Journal of cell science, Jg. 118 (2005-03-01), Heft Pt 5, S. 873-87 |
Veröffentlichung: | Cambridge : Company of Biologists ; <i>Original Publication</i>: London., 2005 |
Medientyp: | academicJournal |
ISSN: | 0021-9533 (print) |
DOI: | 10.1242/jcs.01634 |
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