A mutation in the essential gene gmk (encoding guanlyate kinase) generates a requirement for adenine at low temperature in Salmonella enterica.
In: Journal of bacteriology, Jg. 185 (2003-11-01), Heft 22, S. 6732-5
academicJournal
Zugriff:
In Salmonella enterica serovar Typhimurium, gmk encodes guanylate kinase, an essential enzyme involved in the synthesis and salvage of guanine nucleotides. Here we report the isolation of a mutation in gmk that results in a nutritional requirement for adenine at low temperature. Comparisons of kinetic parameters from the wild-type and mutant Gmk enzymes revealed that the mutant enzyme had a more than 20-fold-higher Km for ATP than the wild-type enzyme. The growth dependence of the mutant on temperature and/or adenine could not be explained as a direct result of this kinetic difference. We propose a model in which previously described regulatory effects of GMP are responsible for these phenotypes.
Titel: |
A mutation in the essential gene gmk (encoding guanlyate kinase) generates a requirement for adenine at low temperature in Salmonella enterica.
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Autor/in / Beteiligte Person: | Beck, BJ ; Huelsmeyer, M ; Paul, S ; Downs, DM |
Zeitschrift: | Journal of bacteriology, Jg. 185 (2003-11-01), Heft 22, S. 6732-5 |
Veröffentlichung: | Washington, DC : American Society for Microbiology, 2003 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
DOI: | 10.1128/JB.185.22.6732-6735.2003 |
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