Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites.
In: Journal of cell science, Jg. 115 (2002-12-15), Heft Pt 24, S. 4777-86
academicJournal
Zugriff:
PINCH, integrin-linked kinase (ILK) and calponin homology-containing ILK-binding protein (CH-ILKBP) form a ternary complex that plays crucial roles at cell-extracellular matrix adhesion sites. To understand the mechanism underlying the complex formation and recruitment to cell-adhesion sites we have undertaken a combined structural, mutational and cell biological analysis. Three-dimensional structure-based point mutations identified specific PINCH and ILK sites that mediate the complex formation. Analyses of the binding defective point mutants revealed that the assembly of the PINCH-ILK-CH-ILKBP complex is essential for their localization to cell-extracellular matrix adhesion sites. The formation of the PINCH-ILK-CH-ILKBP complex precedes integrin-mediated cell adhesion and spreading. Furthermore, inhibition of protein kinase C, but not that of actin polymerization, inhibited the PINCH-ILK-CH-ILKBP complex formation, suggesting that the PINCH-ILK-CH-ILKBP complex likely serves as a downstream effector of protein kinase C in the cellular control of focal adhesion assembly. Finally, we provide evidence that the formation of the PINCH-ILK-CH-ILKBP complex, while necessary, is not sufficient for ILK localization to cell-extracellular matrix adhesion sites. These results provide new insights into the molecular mechanism underlying the assembly and regulation of cell-matrix adhesion structures.
Titel: |
Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites.
|
---|---|
Autor/in / Beteiligte Person: | Zhang, Y ; Chen, K ; Tu, Y ; Velyvis, A ; Yang, Y ; Qin, J ; Wu, C |
Zeitschrift: | Journal of cell science, Jg. 115 (2002-12-15), Heft Pt 24, S. 4777-86 |
Veröffentlichung: | Cambridge : Company of Biologists ; <i>Original Publication</i>: London., 2002 |
Medientyp: | academicJournal |
ISSN: | 0021-9533 (print) |
DOI: | 10.1242/jcs.00166 |
Schlagwort: |
|
Sonstiges: |
|