ICK is essential for cell type-specific ciliogenesis and the regulation of ciliary transport.
In: EMBO Journal, Jg. 33 (2014-06-02), Heft 11, S. 1227-1242
academicJournal
Zugriff:
Cilia and flagella are formed and maintained by intraflagellar transport ( IFT) and play important roles in sensing and moving across species. At the distal tip of the cilia/flagella, IFT complexes turn around to switch from anterograde to retrograde transport; however, the underlying regulatory mechanism is unclear. Here, we identified ICK localization at the tip of cilia as a regulator of ciliary transport. In ICK-deficient mice, we found ciliary defects in neuronal progenitor cells with Hedgehog signal defects. ICK-deficient cells formed cilia with mislocalized Hedgehog signaling components. Loss of ICK caused the accumulation of IFT- A, IFT- B, and BBSome components at the ciliary tips. In contrast, overexpression of ICK induced the strong accumulation of IFT- B, but not IFT- A or BBSome components at ciliary tips. In addition, ICK directly phosphorylated Kif3a, while inhibition of this Kif3a phosphorylation affected ciliary formation. Our results suggest that ICK is a Kif3a kinase and essential for proper ciliogenesis in development by regulating ciliary transport at the tip of cilia. [ABSTRACT FROM AUTHOR]
Titel: |
ICK is essential for cell type-specific ciliogenesis and the regulation of ciliary transport.
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Autor/in / Beteiligte Person: | Chaya, Taro ; Omori, Yoshihiro ; Kuwahara, Ryusuke ; Furukawa, Takahisa |
Zeitschrift: | EMBO Journal, Jg. 33 (2014-06-02), Heft 11, S. 1227-1242 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 0261-4189 (print) |
DOI: | 10.1002/embj.201488175 |
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