Protein Tyrosine Kinase Wee1B Is Essential for Metaphase II Exit in Mouse Oocytes.
In: Science, Jg. 332 (2011-04-22), Heft 6028, S. 462-465
academicJournal
Zugriff:
Waves of cyclin synthesis and degradation regulate the activity of Cdc2 protein kinase during the cell cycle. Cdc2 inactivation by Wee1B-mediated phosphorylation is necessary for arrest of the oocyte at G2-prophase, but it is unclear whether this regulation functions later during the metaphase-to-anaphase transition. We show that reactivation of a Wee1B pathway triggers the decrease in Cdc2 activity during egg activation. When Wee1B is down-regulated, oocytes fail to form a pronucleus in response to Ca2+ signals. Calcium-calmodulin-dependent kinase II (CaMKII) activates Wee1B, and CaMKII-driven exit from metaphase II is inhibited by Wee1B down-regulation, demonstrating that exit from metaphase requires not only a proteolytic degradation of cyclin B but also the inhibitory phosphorylation of Cdc2 by Wee1B. [ABSTRACT FROM AUTHOR]
Titel: |
Protein Tyrosine Kinase Wee1B Is Essential for Metaphase II Exit in Mouse Oocytes.
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Autor/in / Beteiligte Person: | Jeong Su Oh ; Susor, Andrej ; Conti, Marco |
Zeitschrift: | Science, Jg. 332 (2011-04-22), Heft 6028, S. 462-465 |
Veröffentlichung: | 2011 |
Medientyp: | academicJournal |
ISSN: | 0036-8075 (print) |
DOI: | 10.1126/science.1199211 |
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