CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca<superscript>2+</superscript> Entry.
In: Science, Jg. 312 (2006-05-26), Heft 5777, S. 1220-1223
academicJournal
Zugriff:
Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery. [ABSTRACT FROM AUTHOR]
Titel: |
CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca<superscript>2+</superscript> Entry.
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Autor/in / Beteiligte Person: | Vig, M. ; Peinelt, C. ; Beck, A. ; Koomoa, D. L. ; Rabah, D. ; Koblan-Huberson, M. ; Kraft, S. ; Turner, H. ; Fleig, A. ; Penner, R. ; Kinet, J. P. |
Zeitschrift: | Science, Jg. 312 (2006-05-26), Heft 5777, S. 1220-1223 |
Veröffentlichung: | 2006 |
Medientyp: | academicJournal |
ISSN: | 0036-8075 (print) |
DOI: | 10.1126/science.1127883 |
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