髓过氧化物酶-463G/A 基因多态性与彝族原发性高血压的相关性.

Objective To study the correlation between the polymorphism of myeloperoxidase-463G/A gene and essential hypertension of Yi nationality in Yunnan Province. Methods The PCR-RFLP technique was used to detect the MPO-463G/A gene polymorphism in 86 Yi hypertensive patients and 100 normal Yi people in Shuanghe Township and Yunnan Province collected by the First Affiliated Hospital of Kunming Medical University from January 2007 to November 2008. The frequencies of the GG, GA, and AA genotypes and the G and A allele frequencies were calculated, followed by association analysis. Results The genotype frequencies of MPO gene-463G/A polymorphism in EH group and control group GG were 66.28%，52.00%，GA+AA were 33.72%, 48.00%, respectively. The frequencies of allele G were 79.65% and 69.50%, and the frequencies of allele A were 20.35% and 30.50%, respectively. There were statistically significant differences in genotype frequency and gene frequency between the two groups（P < 0.05）. The genotype frequencies of GG and GA+AA were 74.50% and 49.30% in female EH group and 25.50% and 50.70% in femal control group. The allele frequencies of G and A were 85.50% and 69.00% in EH group and 14.50% and 31.00% in control group. The differences were statistically significant（P < 0.05）. The frequency of A gene in female EH group was significantly lower than that in female control group. There was no significant difference in genotype frequency and gene frequency between EH group and control group in Yi male（P > 0.05）. Conclusion MPO gene-463G/A polymorphism is related to essential hypertension in Yunnan Yi women and is a susceptibility gene for hypertension in Yi women. The expression of the A allele at this locus has a protective effect during the development of EH in Yi women. [ABSTRACT FROM AUTHOR]

目的 研究髓过氧化物酶（MPO）-463G/A 基因的多态性与云南彝族原发性高血压（EH）的相关性。 方法 应用聚合酶链反应-限制性片段长度多态性（PCR-RELP）技术，检测 2007 年 1 月至 2008 年 11 月由昆明 医科大学第一附属医院采自云南省双河乡彝族高血压患者（EH 组，n = 86）和彝族正常人（对照组，n = 100）的 MPO-463G/A 基因多态性，并计算 GG、GA、AA 基因型频率及 G、A 基因频率，再进行关联分析。结果 在 EH 组和对照组中，MPO 基因-463G/A 多态的基因型频率 GG 为 66.28%、52.00%，GA+AA 为 33.72%、48.00%， 等位基因 G 频率为 79.65%、69.50%，等位基因 A 频率为 20.35%、30.50%，2 组基因型频率和基因频率分别相 比，差异均具有统计学意义（P < 0.05）。彝族女性 EH 组和女性对照组中 GG 和 GA+AA 基因型频率分别为 74.50%、49.30% 和 25.50%、50.70%，等位基因 G 和 A 的频率分别为 85.50%、69.00% 和 14.50%、31.00%，2 组基因型频率和基因频率分别相比，差异均具有统计学意义（P <0.05），EH 组 A 基因频率明显低于对照组。彝 族男性 EH 组和男性对照组的基因型频率和基因频率分别比较，差异均无统计学意义（P > 0.05）。结论 MPO 基 因-463G/A 多态与云南彝族女性原发性高血压具有相关性，是彝族女性高血压的易感基因。该位点 A 基因的表 达在彝族女性 EH 的发生过程中起保护性作用。 [ABSTRACT FROM AUTHOR]