Essential amino acid starvation induces cell cycle arrest, autophagy, and inhibits osteogenic differentiation in murine osteoblast.

This study investigates the effects of essential amino acid (EAA) starvation on murine osteoblasts cells and the underlying mechanisms. We performed and observed the cell proliferation, autophagy, and osteogenic differentiation under deprivation of EAA in vitro. The results showed that EAA starvation resulted in cell cycle arrest via phosphorylation of the MAPK signaling pathway, leading to inhibition of cell proliferation and osteogenic differentiation. Additionally, the LKB1-AMPK signaling pathway was also found to be phosphorylated, inducing autophagy. These findings highlight the significant role of EAA in regulating cellular processes. Furthermore, this study contributes to our understanding of the effects of nutrient deprivation on cellular physiology and may aid in the development of novel therapeutic strategies for diseases associated with amino acid metabolism. • This study explores the cellular changes that occur in murine osteoblasts during the starvation of essential amino acids (EAA) and elucidates the underlying mechanisms. • The findings reveal that EAA starvation leads to cell cycle G2/M arrest in osteoblasts through phosphorylation of the MAPK signaling pathway, resulting in inhibited cell proliferation and osteogenic differentiation. • Moreover, the study demonstrates that the LKB1-AMPK signaling pathway is phosphorylated under EAA deprivation, triggering autophagy. • These findings enhance our understanding of the physiological impact of nutrient deprivation and EAA starvation on bone homeostasis and new formation. [ABSTRACT FROM AUTHOR]