The association of ODF4 with AK1 and AK2 in mice is essential for fertility through its contribution to flagellar shape.
In: Scientific Reports, Jg. 13 (2023-02-20), Heft 1, S. 1-15
academicJournal
Zugriff:
Normal sperm flagellar shape and movement are essential for fertilization. The integral protein outer dense fiber 4 (ODF4) localizes to ODFs, but its function remains unclear. Adenylate kinase (AK) is a phosphotransferase that catalyzes the interconversion and controls the concentration equilibrium of adenine nucleotides. AK shuttles ATP to energy-consuming sites. Here, we report on the relationship of flagellar shape and movement with ODF4, AK1 and AK2 by using Odf4-deletion (Odf4−/−) mice. Soluble ODF4 is coimmunoprecipitated with AK1 and AK2 in Odf4+/+ spermatozoa. ODF4, AK1 and AK2 localize to whole flagella (plasmalemma, mitochondria, ODFs, and residual cytoplasmic droplets (CDs)), principal pieces, and midpieces, respectively. Odf4−/− sperm flagella lose ODF4 and reduce AK1 and AK2 but produce ATP. The flagellum is bent (hairpin flagellum) with a large CD in the midpiece. There is no motility in the midpiece, but the principal piece is motile. Odf4−/− spermatozoa progress backward and fail to ascend in the uterus. Thus, Odf4−/− males are infertile owing to abnormal flagellar shape and movement caused mainly by the loss of ODF4 with AK1 and AK2. This study is supported by the rescue experiment; the abnormalities and male infertility caused by Odf4 deletion were reversed by Odf4 restoration. [ABSTRACT FROM AUTHOR]
Titel: |
The association of ODF4 with AK1 and AK2 in mice is essential for fertility through its contribution to flagellar shape.
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Autor/in / Beteiligte Person: | Ito, Chizuru ; Makino, Tsukasa ; Mutoh, Tohru ; Kikkawa, Masahide ; Toshimori, Kiyotaka |
Zeitschrift: | Scientific Reports, Jg. 13 (2023-02-20), Heft 1, S. 1-15 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-023-28177-z |
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