Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.
In: Molecular & Cellular Biology, Jg. 38 (2018-12-15), Heft 24, S. 1-16
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Zugriff:
Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA and mRNA expression relies on a set of common factors, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Here, we investigated a potential role for the Mediator complex, essential for mRNA gene transcription, in sn/ snoRNA gene transcription. We found that Mediator maps to sn/snoRNA gene regulatory regions and that rapid depletion of the essential structural subunit Med14 strongly reduces RNAPII and TFIIB occupancy as well as nascent transcription of sn/ snoRNA genes. Deletion of Med3 and Med15, subunits of the activator-interacting Mediator tail module, does not affect Mediator recruitment to or RNAPII and TFIIB occupancy of sn/snoRNA genes. Our analyses suggest that Mediator promotes PIC formation and transcription at sn/snoRNA genes, expanding the role of this critical regulator beyond its known functions in mRNA gene transcription and demonstrating further mechanistic similarity between the transcription of mRNA and sn/snoRNA genes. [ABSTRACT FROM AUTHOR]
Titel: |
Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.
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Autor/in / Beteiligte Person: | Tourigny, Jason P. ; Saleh, Moustafa M. ; Schumacher, Kenny ; Devys, Didier ; Zentnera, Gabriel E. |
Zeitschrift: | Molecular & Cellular Biology, Jg. 38 (2018-12-15), Heft 24, S. 1-16 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 0270-7306 (print) |
DOI: | 10.1128/MCB.00296-18 |
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