Minor zygotic gene activation is essential for mouse preimplantation development.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 115 (2018-07-17), Heft 29, S. E6780- (9S.)
academicJournal
Zugriff:
In mice, transcription initiates at the mid-one-cell stage and transcriptional activity dramatically increases during the two-cell stage, a process called zygotic gene activation (ZGA). Associated with ZGA is a marked change in the pattern of gene expression that occurs after the second round of DNA replication. To distinguish ZGA before and after the second-round DNA replication, the former and latter are called minor and major ZGA, respectively. Although major ZGA are required for development beyond the two-cell stage, the function of minor ZGA is not well understood. Transiently inhibiting minor ZGA with 5, 6-dichloro-1-ß-D-ribofuranosyl-benzimidazole (DRB) resulted in the majority of embryos arresting at the two-cell stage and retention of the H3K4me3 mark that normally decreases. After release from DRB, at which time major ZGA normally occurred, transcription initiated with characteristics of minor ZGA but not major ZGA, although degradation of maternal mRNA normally occurred. Thus, ZGA occurs sequentially starting with minor ZGA that is critical for the maternal-to-zygotic transition. [ABSTRACT FROM AUTHOR]
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Minor zygotic gene activation is essential for mouse preimplantation development.
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Autor/in / Beteiligte Person: | Abe, Ken-ichiro ; Funaya, Satoshi ; Tsukioka, Dai ; Kawamura, Machika ; Suzuki, Yutaka ; Suzuki, Masataka G. ; Schultz, Richard M. ; Aoki, Fugaku |
Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 115 (2018-07-17), Heft 29, S. E6780- (9S.) |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 0027-8424 (print) |
DOI: | 10.1073/pnas.1804309115 |
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