The WD-repeat protein Pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex.
In: EMBO Journal, Jg. 19 (2000), Heft 1, S. 37-47
academicJournal
Zugriff:
In the yeast Saccharomyces cerevisiae, pre-mRNA 3′-end processing requires six factors: cleavage factor IA (CF IA), cleavage factor IB (CF IB), cleavage factor II (CF II), polyadenylation factor I (PF I), poly(A) polymerase (Pap1p) and poly(A)-binding protein I (Pab1p). We report the characterization of Pfs2p, a WD-repeat protein previously identified in a multiprotein complex carrying PF I-Pap1p activity. The 3′-end-processing defects of pfs2 mutant strains and the results of immunodepletion and immunoinactivation experiments indicate an essential function for Pfs2p in cleavage and polyadenylation. With a one-step affinity purification method that exploits protein A-tagged Pfs2p, we showed that this protein is part of a CF II-PF I complex. Pull-down experiments with GST fusion proteins revealed direct interactions of Pfs2p with subunits of CF II-PF I and CF IA. These results show that Pfs2p plays an essential role in 3′-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex. [ABSTRACT FROM AUTHOR]
Titel: |
The WD-repeat protein Pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex.
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Autor/in / Beteiligte Person: | Ohnacker, Martin ; Barabino, Silvia M. L. ; Preker, Pascal J. ; Keller, Walter |
Zeitschrift: | EMBO Journal, Jg. 19 (2000), Heft 1, S. 37-47 |
Veröffentlichung: | 2000 |
Medientyp: | academicJournal |
ISSN: | 0261-4189 (print) |
DOI: | 10.1093/emboj/19.1.37 |
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