The essential role of profilin in the assembly of actin for microspike formation.
In: EMBO Journal, Jg. 17 (1998-11-15), Heft 22, S. 6516-6526
academicJournal
Zugriff:
Profilin was first identified as an actin monomer binding protein; however, recent reports indicate its involvement in actin polymerization. To date, there is no direct evidence of a functional role in vivo for profilin in actin cytoskeletal reorganization. Here, we prepared a profilin mutant (H119E) defective in actin binding, but retaining the ability to bind to other proteins. This mutant profilin I suppresses actin polymerization in microspike formation induced by N-WASP, the essential factor in microspike formation. Profilin associates both in vivo and in vitro with N-WASP at prolinerich sites different from those to which Ash/Grb2 binds. This association between profilin and N-WASP is required for N-WASP-induced efficient microspike elongation. Moreover, we succeeded in reconstituting microspike formation in permeabilized cells using profilin I combined with N-WASP and its regulator, Cdc42. These findings provide the first evidence that profilin is a key molecule linking a signaling network to rapid actin polymerization in microspike formation. [ABSTRACT FROM AUTHOR]
Titel: |
The essential role of profilin in the assembly of actin for microspike formation.
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Autor/in / Beteiligte Person: | Suetsugu, Shiro ; Miki, Hiroaki ; Takenawa, Tadaomi |
Zeitschrift: | EMBO Journal, Jg. 17 (1998-11-15), Heft 22, S. 6516-6526 |
Veröffentlichung: | 1998 |
Medientyp: | academicJournal |
ISSN: | 0261-4189 (print) |
DOI: | 10.1093/emboj/17.22.6516 |
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