A non-chromosomal factor allows viability of Schizosaccharomyces pombe lacking the essential chaperone calnexin.
In: Journal of Cell Science, Jg. 117 (2004-02-29), Heft 6, S. 907-918
academicJournal
Zugriff:
Calnexin is a molecular chaperone playing key roles in protein folding and the quality control of this process in the endoplasmic reticulum. We, and others, have previously demonstrated that cnx1+, the gene encoding the calnexin homologue in Schizosaccharomyces pombe, is essential for viability. We show that a particular cnx1 mutant induces a novel mechanism allowing the survival of S. pombe cells in the absence of calnexin/Cnx1p. Calnexin independence is dominant in diploid cells and is inherited in a non-Mendelian manner. Remarkably, this survival pathway, bypassing the necessity for calnexin, can be transmitted by transformation of cell extracts into a wild-type naive strain, thus implicating a non-chromosomal factor. Nuclease and UV treatments of cells extracts did not obliterate transmission of calnexin independence by transformation. However, protease digestion of extracts did reduce the appearance of calnexin-independent cells, indicating that a protein element is required for calnexin-less viability. We discuss a model in which this calnexin-less survival mechanism would be activated and perpetuated by a protein component acting as a genetic element. [ABSTRACT FROM AUTHOR]
Titel: |
A non-chromosomal factor allows viability of Schizosaccharomyces pombe lacking the essential chaperone calnexin.
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Autor/in / Beteiligte Person: | Collin, Philippe ; Beauregard, Pascale B. ; Elagöz, Aram ; Rokeach, Luis A. |
Zeitschrift: | Journal of Cell Science, Jg. 117 (2004-02-29), Heft 6, S. 907-918 |
Veröffentlichung: | 2004 |
Medientyp: | academicJournal |
ISSN: | 0021-9533 (print) |
DOI: | 10.1242/jcs.00943 |
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