A Broad Range of Conformations Contribute to the Solution Ensemble of the Essential Splicing Factor U2AF<superscript>65 </superscript>.
In: Biochemistry, Jg. 51 (2012-07-03), Heft 26, S. 5223-5225
academicJournal
Zugriff:
U2AF65 is essential for pre-mRNA splicing In most eukaryotes. Two consecutive RNA recognition motifs (RRM) of U2AF65 recognize a polypyrimidine tract at the 3' splice Site. Here, we use small-angle X-ray scattering to demonstrate that the tandem U2AF65 RRMs exhibit a broad range of conformations in the solution ensemble. The majority of U2AF65 conformations exhibit few contacts between the RRMs, such as observed in the crystal structure. A subpopulation adopts tight inter-RRM contacts, such as independently reported based on parsmagnetic relaxation enhancements. These complementary structural methods demonstrate that diverse splice sites have the opportunity to select compact or extended inter-RRM proximities from the U2AF65 conformational pool. [ABSTRACT FROM AUTHOR]
Titel: |
A Broad Range of Conformations Contribute to the Solution Ensemble of the Essential Splicing Factor U2AF<superscript>65 </superscript>.
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Autor/in / Beteiligte Person: | Jenkins, Jermaine L. ; Laird, Kholiswa M. ; Kielkop, Clara L. |
Zeitschrift: | Biochemistry, Jg. 51 (2012-07-03), Heft 26, S. 5223-5225 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0006-2960 (print) |
DOI: | 10.1021/bi300277t |
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