ODREĐIVANJE ANETOLA U ETARSKOM ULJU I EKSTRAKTIMA PLODA MORAČA (Foeniculi fructus).

The method for anethole determination in the essential oil of fennel fruit (Foeniculi fructus), as well as in fennel extracts, using Thin Layer Chromatography (TLC) and UV-spectrophotometry, was developed. The essential oil was isolated by hydrodistillation. The ex tracts of fennel fruit were obtained by extraction using 95% ethanol and by SFE method using super critical CO2 (pressure 100 bar, temperature 40°C, extraction time 4 h). A successful separation of anethole was obtained by using the solvent system cyclohexan-ethylacetate (98:2, v/v) as a mobile phase in TLC. The anethole content in the essential oil of 77.82% (w/w), as well as in the ex tract obtained by 95 % ethanol (0.381%, w/v) and CO2-extract (62.30%, w/w) was determined. [ABSTRACT FROM AUTHOR]

Razrađen je metod za određivanje anetola u etarskom ulju i ekstraktima droge -- ploda morača (Foeniculi fructus), primenom hromatografije na tankom sloju silikagela i UV spektrofotometrije. Etarsko ulje je dobijeno destilacijom droge pomoču vodene pare, a za ekstrakciju je primenjen 95% etanol i ugljendioksid u superkritičnom stanju (pritisak 100 bar, temperatura 40°C, vreme ekstrakcije 4h). Razdvajanje anetola je izvršeno primenom rastvaračkog sistema ciklohek-san--etilacetat (98:2,v/v) kao pokretne faze. Nađeno je da sadržaj anetola iznosi: u etarskom ulju 77,22 % (m/m), 95% etanolnom ekstraktu 0,381 % (m/v) i CO2-ekstraktu 62,30 % (m/m). [ABSTRACT FROM AUTHOR]