Genetic Interaction mapping of essential genes in Saccharomyces cerevisiae.
In: FASEB Journal, Jg. 21 (2007-04-30), Heft 6, S. A1004
academicJournal
Zugriff:
Genetic interactions measure how mutation in one gene contributes to the phenotype afflicted with a second gene mutation. Previous genetic mapping of Saccharomyces cerevisiae using E-MAPs (epistatic miniarray profile) have revealed great insight into the functions of previously characterized and uncharacterized non-essential genes. However, systematic genetic analysis of essential genes has been hard to achieve because deletion of essential genes leads to cell death. We have developed a new technique, termed DAmP (Decreased Abundance by mRNA Perturbation), where hypomorphs of essential genes are created by disruption of their 3'UTR sequences. We have generated a quantitative genetic map of 96 essential genes involved in various aspect of chromosome functioning and regulation using DAmP and E-MAP technology to reveal genetic interactions within this essential biological process. [ABSTRACT FROM AUTHOR]
Titel: |
Genetic Interaction mapping of essential genes in Saccharomyces cerevisiae.
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Autor/in / Beteiligte Person: | Huang, Jiahao ; Krogan, Nevan ; Schuldiner, Maya |
Zeitschrift: | FASEB Journal, Jg. 21 (2007-04-30), Heft 6, S. A1004 |
Veröffentlichung: | 2007 |
Medientyp: | academicJournal |
ISSN: | 0892-6638 (print) |
DOI: | 10.1096/fasebj.21.6.a1004-c |
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