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Dach1 is essential for maintaining normal mature podocytes.
In: PLoS ONE, Jg. 19 (2024-05-28), Heft 5, S. 1-15
Online
academicJournal
Zugriff:
Dach1 is highly expressed in normal podocytes, but this expression rapidly disappears after podocyte injury. To investigate the role of Dach1 in podocytes in vivo, we analyzed global, podocyte-specific, and inducible Dach1 knockout mice. Global Dach1 knockout (Dach1-/-) mice were assessed immediately after birth because they die within a day. The kidneys of Dach1-/- mice were slightly smaller than those of control mice but maintained a normal structure and normal podocyte phenotypes, including ultrastructure. To study the role of Dach1 in mature podocytes, we generated Dach1 knockout mice by mating Dach1fl/fl mice with Nphs1-Cre or ROSA-CreERT2 mice. Due to inefficient Cre recombination, only a small number of podocytes lacked Dach1 staining in these mice. However, all eleven Nphs1-Cre/Dach1fl/fl mice displayed abnormal albuminuria, and seven (63%) of them developed focal segmental glomerulosclerosis. Among 13 ROSA-CreERT2/Dach1fl/fl mice, eight (61%) exhibited abnormal albuminuria after treatment with tamoxifen, and five (38%) developed early sclerotic lesions. These results indicate that while Dach1 does not determine the fate of differentiation into podocytes, it is indispensable for maintaining the normal integrity of mature podocytes. [ABSTRACT FROM AUTHOR]
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Dach1 is essential for maintaining normal mature podocytes.
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Autor/in / Beteiligte Person: | Tanaka, Keiko ; Hayasaka, Haruko ; Matsusaka, Taiji |
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Zeitschrift: | PLoS ONE, Jg. 19 (2024-05-28), Heft 5, S. 1-15 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0303910 |
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