An upcycled fraction of Melaleuca alternifolia essential oil regenerates the skin through the skin melatonin pathway and improves sleep quality.

Background: Sleep is one of the most important factors affecting overall health. During the night, the skin repairs damage caused by daily stresses. Melatonin plays a key role in this process. Toxins are removed, and cellular repair and growth hormone production are increased. Inter alia, this also decreases signs of intrinsic aging. Aims: The current study was intended to demonstrate the impact of a unique fraction of Melaleuca alternifolia (FMA) essential oil, on sleep and skin quality. Methods: The effect of FMA was investigated in vitro on skin cells, evaluating its antioxidant and anti‐inflammatory properties, and in an ex‐vivo study on human skin biopsies treated with FMA following stress induction. In addition, two clinical studies were performed on volunteers with life‐style‐related sleep complaints. In one study, sleep was measured using a noncontact monitoring device (SleepScore Labs, Max). A second study was conducted to assess skin anti‐aging effects. Results: In vitro application of FMA reduced IL‐8 and reactive oxygen species (ROS) generation in skin cells. This was confirmed ex vivo through a decrease in inflammatory markers and an increase in antioxidant enzymes after stress induction. Interestingly, FMA also upregulated melatonin‐associated genes. Real‐world sleep tracking revealed that FMA significantly improved sleep quality, relative to unscented control. In vivo applications also showed a reduction in signs of aging. Conclusion: These results provide initial data to suggest that this unique FMA delivers skin anti‐aging benefits via a two‐pronged mode of action, improving sleep quality, and reducing skin inflammatory and oxidative stress. [ABSTRACT FROM AUTHOR]