Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam.
In: Ciência Rural, Jg. 54 (2024-05-01), Heft 5, S. 1-12
Online
academicJournal
Zugriff:
This study determined the extraction conditions, chemical composition, and antimicrobial activities of the essential oils of Citrus nobilis. The results illustrated that soaking in the 9% (w/v) NaCl solution for 2 h with a sample and solvent ratio of 1:3 (w/v) and a subsequent extraction time of 45 min yielded the highest extraction efficiency, reaching 3.66% (w/w). The main chemical components of the essential oils were limonene (90.42%), ß-myrcene (4.7%), and a-pinene (1.22%). The minimum inhibitory concentration (MIC) at the density of 5x104, 5x105, and 5x106 cells/mL of Escherichia coli were 100, 125, and 125 mg/mL, respectively; for Staphylococcus aureus were 75, 100, and 125 mg/mL, respectively; and for Bacillus cereus were 50, 75, and 100 mg/mL, respectively. The minimum bactericidal concentration (MBC) at densities of 5x104, 5x105, 5x106 cells/mL of E. coli were 175, 225, 225 mg/mL, S. aureus were 150, 200, 225 mg/mL, and B. cereus were 125, 175, 200 mg/mL, respectively. The inhibitory activities of Aspergillus flavus of orange essential oils according to the agar dilution method at concentrations of 0.025-0.1%) on the 5th day were 8.84-30.61%. [ABSTRACT FROM AUTHOR]
Este estudo teve como objetivo determinar as condições de extração, composição química e atividades antimicrobianas dos óleos essenciais de Citrus nobilis. Os resultados demonstram que a imersão na solução de NaCl a 9% (p/v) por 2 h com uma proporção de amostra e solvente de 1:3 (p/v) e um tempo de extração subsequente de 45 min produziu a maior eficiência de extração, atingindo 3,66 % (p/p). Os principais componentes químicos dos óleos essenciais foram limoneno (90,42%), ß-mirceno (4,7%) e a-pineno (1,22%). A concentração inibitória mínima (CIM) na densidade de 5x104, 5x105 e 5x106 células/mL de Escherichia coli foram 100, 125 e 125 mg/mL, respectivamente, e para Staphylococcus aureus foram 75, 100 e 125 mg/mL, respectivamente, e para Bacillus cereus foram 50, 75 e 100 mg/mL, respectivamente. A concentração bactericida mínima (MBC) em densidades de 5x104, 5x105, 5x106 células/mL de E. coli foram 175, 225, 225 mg/mL, S. aureus foram 150, 200, 225 mg/mL e B. cereus foram 125, 175, 200 mg/mL, respectivamente. As atividades inibitórias de Aspergillus flavus dos óleos essenciais de laranja, de acordo com o método de diluição em ágar nas concentrações 0,025-0,1%, no dia cinco foram 8,84-30,61%. [ABSTRACT FROM AUTHOR]
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Extraction and evaluation of antimicrobial activities of essential oils from orange peel (Citrus nobilis) grown in Can Tho City, Vietnam.
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Autor/in / Beteiligte Person: | Ly Thi Thuy Duyen ; Khong Hoang Thang ; Luu Minh Chau ; Tran Thi Giang ; Nguyen Ngoc Thanh ; Bui Hoang Dang Long ; Huynh Xuan Phong |
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Zeitschrift: | Ciência Rural, Jg. 54 (2024-05-01), Heft 5, S. 1-12 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0103-8478 (print) |
DOI: | 10.1590/0103-8478cr20230240 |
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