OsGSTU34, a Bz2-like anthocyanin-related glutathione transferase transporter, is essential for rice (Oryza sativa L.) organs coloration.

Anthocyanins are a flavonoid compound known as one of the most important chromogenic substances. They play several functions, including health promotion and sustaining plants during adverse conditions. They are synthesized at the endoplasmic reticulum and sequestered in the vacuole. In this work, we generated knock-out lines of OsGSTU34, a glutathione transporter's tau gene family, with no transgene line and off-target through CRISPR/Cas9 mutagenesis and highlighted the loss of pigmentation in rice flowers, leaves, stems, shoots, and caryopsis. The anthocyanin quantification in the wild-type BLWT and mutant line BLG34-8 caryopsis showed that cyanidin-3-O-glucoside (C3G) and peonidin-3-O-glucoside (P3G) were almost undetectable in the mutant line. A tandem mass tag (TMT) labeling proteomic analysis was conducted to elucidate the proteomic changes in the BLWT and BLG34-8. The result revealed that 1175 proteins were altered, including 408 that were down-regulated and 767 that were upregulated. The accumulation of the OsGSTU34 -related protein (Q8L576), along with several anthocyanin-related proteins, was down-regulated. The enrichment analysis showed that the down-regulated proteins were enriched in different pathways, among which the phenylpropanoid biosynthesis pathway, flavonoid biosynthesis metabolites, and anthocyanin biosynthesis pathway. Protein interaction network prediction revealed that glutathione-S-transferase (Q8L576) was connected to the proteins involved in the flavonoid and anthocyanin biosynthesis pathways, such as flavanone 3-dioxygenase 1 (Q7XM21), leucoanthocyanidin dioxygenase 1 (Q93VC3), 4-coumarate-CoA ligase 2 (Q42982), phenylalanine ammonia-lyase (P14717), chalcone synthase 1 (Q2R3A1), and 4-coumarate-CoA ligase 5 (Q6ZAC1). However, the expression of the most important anthocyanin biosynthesis gene was not altered, suggesting that only the transport mechanism was affected. Our findings highlight new insight into the anthocyanin pigmentation in black rice and provide new perspectives for future research. CRISPR/Cas9 gene editing targeting OsGSTU34 , a Bz2-like anthocyanin-related glutathione transferase transporter, greatly affects pigmentation in different parts of black rice. The two main anthocyanins in black rice, identified as cyanidin-3-O-glucoside (C3G) and peonidin-3-O-glucoside (P3G), were almost undetectable in the mutant line Osgstu34. [Display omitted] • Anthocyanins are phytochemicals responsible for black rice (O. sativa L.) pigmentation. • Cyanidin-3-O-glucoside (C3G) and peonidin-3-O-glucoside (P3G) are the main anthocyanins in black rice. • The knockout of OsGSTU34 causes loss of pigmentation in different parts of black rice. • The amounts of C3G and P3G were almost undetectable in the mutant-type Osgstu34. • The loss of function of OsGSTU34 may not affect anthocyanin biosynthesis but only accumulation. [ABSTRACT FROM AUTHOR]