CYP11B2 基因-344C/T 多态与云南双河乡彝族 人群原发性高血压的相关性.

Objective: To investigate the correlation between CYP11B2 gene -344C/T polymorphism and essential hypertension in Yi people of Yunnan Shuanghe Township. Methods: We collected peripheral blood of 308 Yi residents of Shuanghe township, Jinning county, Yunnan province, including 132 patients with essential hypertension (EH group) and 176 control group members. The target fragment was amplified by PCR and restriction endonuclease enzymes were used. Identify the genotype, calculate genotype frequency and allele frequency, and perform correlation analysis. Results: The genotype frequency of TT in the EH group/control group was 64.39%/60.23%, CT was 29.55%/32.95%, CC was 6.06%/6.81%, and the allele T frequency was 79.17% /76.70%, the frequency of allele C was 20.83%/23.30%;but there was no significant difference between the two groups (P > 0.05), however, the TT genotype frequency in female patients (70.00%) was higher than that in control group (56.44%) (P < 0.05). In EH group, the genotype frequency TT of male /female patients was 55.77%/70.00%, CT was 32.69%/27.50%, CC was 11.54%/2.50%, and allele T frequency was 72.12%/83.75%. The frequency of allele C was 27.88%/16.25%, and the genotype frequency of TT, CC and allele frequency of the two groups were significantly different (P < 0.05). Conclusion: The distribution of CYP11B2 gene -344C/T polymorphism in Yi people with essential hypertension has gender differences, but it may not be related to essential hypertension in Yi people of Yunnan Shuanghe township. [ABSTRACT FROM AUTHOR]

目的　研究 CYP11B2 基因-344C/T 多态与云南省晋宁县双河乡彝族人群原发性高血压的相关性。 方法　采集 308 例云南省晋宁县双河乡彝族居民外周血，包括 132 例原发性高血压患者（EH 组）和 176 例对照 组成员，提取 DNA 后 PCR 扩增目的片段，限制性内切酶酶切鉴定基因型，计算基因型频率和等位基因频率， 并进行相关性分析。结果　CYP11B2 基因-344C/T 多态在 EH 组/对照组中的基因型频率 TT 为 64.39%/60.23%， CT 为29.55%/32.95%，CC 为6.06%/6.81%，等位基因T 频率为79.17% /76.70%，等位基因C 频率为20.83% /23.30%， 2 组的基因型频率和基因频率相比，差异无统计学意义（P > 0.05），但女性患者中的 TT 基因型频率（70.00%）高 于对照组（56.44%）（P < 0.05）。在 EH 组中，男性患者/女性患者的基因型频率 TT 为 55.77%/70.00%，CT 为 32.69%/ 27.50%，CC 为 11.54%/2.50%，等位基因 T 频率为 72.12%/83.75%，等位基因 C 频率为 27.88%/16.25%，2 组的 TT、CC 基因型频率和等位基因频率相比，差异有统计学意义（P < 0.05）。结论　CYP11B2 基因-344C/T 多态分 布在彝族原发性高血压人群中存在性别差异，但可能与云南省晋宁县双河乡彝族人群原发性高血压发病无关。 [ABSTRACT FROM AUTHOR]