AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
In: Journal of Cell Science, Jg. 133 (2020-12-01), Heft 23, S. 1-11
academicJournal
Zugriff:
Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However, how AGS3 regulates the transport of Cdh1 to the plasma membrane remains undetermined. To investigate this, AGS3 was knocked out using the Cas9-sgRNA system. Both trans-Golgi network protein 46 (TGN46, also known as TGOLN2) and transmembrane p24-trafficking protein 7 (TMED7) were tracked in early mouse embryos by tagging these proteins with a fluorescent protein label. We observed that the majority of the AGS3-edited embryos were developmentally arrested and were fragmented after the four-cell stage, exhibiting decreased accumulation of Cdh1 at the membrane. The trans-Golgi network and TMED7-positive vesicles were also dispersed and were not polarized near the membrane. Additionally, increased Gαi1 (encoded by GNAI1) expression could rescue AGS3- overexpressed embryos. In conclusion, AGS3 reinforces the dynamics of the trans-Golgi network and the transport of TMED7-positive cargo containing Cdh1 to the cell-contact surface during early mouse embryo development. [ABSTRACT FROM AUTHOR]
Titel: |
AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
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Autor/in / Beteiligte Person: | Nie, Zheng-Wen ; Niu, Ying-Jie ; Zhou, Wenjun ; Zhou, Dong-Jie ; Kim, Ju-Yeon ; Cui, Xiang-Shun |
Zeitschrift: | Journal of Cell Science, Jg. 133 (2020-12-01), Heft 23, S. 1-11 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
ISSN: | 0021-9533 (print) |
DOI: | 10.1242/jcs.243238 |
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