Mg<superscript>2+</superscript> Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
In: PLoS Genetics, Jg. 12 (2016-08-26), Heft 8, S. 1-23
Online
academicJournal
Zugriff:
Mg2+ serves as an essential cofactor for numerous enzymes and its levels are tightly regulated by various Mg2+ transporters. Here, we analyzed Caenorhabditis elegans strains carrying mutations in genes encoding cyclin M (CNNM) Mg2+ transporters. We isolated inactivating mutants for each of the five Caenorhabditis elegans cnnm family genes, cnnm-1 through cnnm-5. cnnm-1; cnnm-3 double mutant worms showed various phenotypes, among which the sterile phenotype was rescued by supplementing the media with Mg2+. This sterility was caused by a gonadogenesis defect with severely attenuated proliferation of germ cells. Using this gonadogenesis defect as an indicator, we performed genome-wide RNAi screening, to search for genes associated with this phenotype. The results revealed that RNAi-mediated inactivation of several genes restores gonad elongation, including aak-2, which encodes the catalytic subunit of AMP-activated protein kinase (AMPK). We then generated triple mutant worms for cnnm-1; cnnm-3; aak-2 and confirmed that the aak-2 mutation also suppressed the defective gonadal elongation in cnnm-1; cnnm-3 mutant worms. AMPK is activated under low-energy conditions and plays a central role in regulating cellular metabolism to adapt to the energy status of cells. Thus, we provide genetic evidence linking Mg2+ homeostasis to energy metabolism via AMPK. [ABSTRACT FROM AUTHOR]
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Mg<superscript>2+</superscript> Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
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Autor/in / Beteiligte Person: | Ishii, Tasuku ; Funato, Yosuke ; Hashizume, Osamu ; Yamazaki, Daisuke ; Hirata, Yusuke ; Nishiwaki, Kiyoji ; Kono, Nozomu ; Arai, Hiroyuki ; Miki, Hiroaki |
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Zeitschrift: | PLoS Genetics, Jg. 12 (2016-08-26), Heft 8, S. 1-23 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 1553-7390 (print) |
DOI: | 10.1371/journal.pgen.1006276 |
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